Posts Tagged ‘Methods’

Effect of tetramethylpyrazine on DRG neuron P2X3 receptor involved in transmitting pain after burn

Abstract: A burn is a severe injury, and the resulting pain can be very significant. Currently, opioids are the primary method of pain management, but these drugs have side effects; thus, it is of prime focus to research the mechanisms of pain formation and analgesic drugs.Objective: To investigate the effects of tetramethylpyrazine (TMP) on burn pain mediated by the P2X3 receptor.Methods: First-degree and superficial second-degree burn models were used. The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were measured, and P2X3 receptor expression on nerve terminals in burn-injured skin were detected by immunohistochemistry. The effects of TMP on the P2X receptor agonist-activated currents in freshly isolated burn-injured rat dorsal root ganglion (DRG) neurons w…

Evaluation of the Risk of Systemic Fat Mobilization and Fat Embolus Following Liposuction with Dry and Tumescent Technique: An Experimental Study on Rats

In this study we investigated the risk of systemic fat mobilization and fat embolus in rats following liposuction
using dry and tumescent techniques.

Methods  At the end of the procedures, the rats were sacrificed and specimens were obtained from the lungs, kidneys, liver, brain,
and skin. Histological examinations of the specimens were carried out. Liposuction was not performed in the control group
(n = 8), but blood and tissue specimens for histological examinations were obtained.

Results  We found signs of fat embolus in both blood specimens and histological examinations of tissue samples in the study groups.
However, the results of the examinations were normal in the control group. Although there were no fat particles in the blood
before…

Evaluation of the Risk of Systemic Fat Mobilization and Fat Embolus Following Liposuction with Dry and Tumescent Technique: An Experimental Study on Rats

In this study we investigated the risk of systemic fat mobilization and fat embolus in rats following liposuction
using dry and tumescent techniques.

Methods  At the end of the procedures, the rats were sacrificed and specimens were obtained from the lungs, kidneys, liver, brain,
and skin. Histological examinations of the specimens were carried out. Liposuction was not performed in the control group
(n = 8), but blood and tissue specimens for histological examinations were obtained.

Results  We found signs of fat embolus in both blood specimens and histological examinations of tissue samples in the study groups.
However, the results of the examinations were normal in the control group. Although there were no fat particles in the blood
before…